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Highly pure MBP-tagged recombinant proteins with MBPTrap HP
- Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes
- Prepacked with Dextrin Sepharose™ High Performance in 1 ml and 5 ml HiTrap™ columns give convenient, time-saving operation and reproducible results
- Physiological conditions and mild elution preserve the activity of the target protein
- Compatible with commonly used aqueous buffers and easily regenerated with 0.5 M NaOH
- Easy scale-up
- Convenient usage with a syringe, peristaltic pump, or chromatography system such as ÄKTAdesign™
MBPTrap™ HP provides convenient affinity purification of recombinant proteins tagged with maltose binding protein (MBP). These columns are prepacked with Dextrin Sepharose High Performance, which has high binding capacity for MBP-tagged proteins. This robust and stable Sepharose based medium enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. MBPTrap HP is easily regenerated using 0.5 M NaOH.
Read more in MBPTrap HP datafile
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